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This assay is designed to detect low-level mutant DNA in the presence of a predominant wild-type DNA background by utilizing the highly sensitive Amplification Refractory Mutation System (ARMS) in combination with TaqMan probe chemistry. The ARMS technology enables selective amplification of specific mutant alleles, while TaqMan probes provide real-time, highly accurate detection and quantification of target mutations. This dual-technology approach significantly enhances analytical performance, allowing reliable identification of rare genetic variants even at very low concentrations. With a demonstrated sensitivity and specificity exceeding 99%, the assay minimizes the risk of false-positive and false-negative results, ensuring precise and reproducible mutation detection. Its high accuracy and robustness make it a valuable tool for molecular diagnostics, clinical research, and precision medicine applications where early and dependable mutation identification is critical for informed decision-making and patient management.

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